TD.1細(xì)胞

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgG1

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

ATCC Number： CRL-2232?

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

TD.1細(xì)胞Designations: TD.1

Depositors: FM Brodsky

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: TD.1細(xì)胞hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human clathrin heavy chain

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Comments: Animals were immunized with the globular N-terminal domain of clathrin heavy chain.

TD.1細(xì)胞Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody is useful for blotting of clathrin heavy chains from a variety of species. It does not react with native clathrin in immunofluorescence or immunoprecipitation assays.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22634: Nathke IS, et al. TD.1細(xì)胞Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68: 899-910, 1992. PubMed: 1547490