Le Ana細(xì)胞
生長狀態(tài)： 貼壁生長
ATCC Number： CRL-1192?
相關(guān)**： 馬凡氏綜合征
數(shù)量： 大量
器官來源： 皮膚
年限： 2 years
運輸方式： 凍存運輸
細(xì)胞形態(tài)： 成纖維樣
是否是腫瘤細(xì)胞： 0
Le Ana細(xì)胞物種來源： 人
Designations: Le Ana
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Le Ana細(xì)胞Marfan syndrome
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Age: 2 years
Gender: female
Comments: The tissue donor suffered from an atypical form of Marfan syndrome.
The patient presented with no eye findings, normal height and severe joint laxity.
Propagation: Le Ana細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Le Ana細(xì)胞Subculture every 7 to 9 days.
