LCL 8664細胞

細胞類型： B**細胞

ATCC Number： CRL-1805?

相關**： **瘤

是否是腫瘤細胞： 0

物種來源： 獼猴

生長狀態： 懸浮生長

運輸方式： 凍存運輸

年限： 5 years

數量： 大量

LCL 8664細胞細胞形態： **樣

Designations: LCL 8664

Depositors: SR Rangan

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Macaca mulatta

Morphology: lymphoblast

Source: Disease: lymphoma

Cell Type: LCL 8664細胞B lymphocyte;

Cellular Products: B lymphotropic herpesvirus

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 5 years

Gender: male

Comments: CAUTION: the cells are infected with and produce a B lymphotropic herpesvirus (RhEBV) and may represent a potential biohazard.

Free RhEBV is present in the supernatant, and rhesus B lymphocyte transforming activity is present in the medium.

About 1% of the cells express virus capsid antigen, and all express the virus related nuclear antigen.

Propagation: LCL 8664細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp5 cells/ml and maintain between 4 X 10 exp5 and 2 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22860: Rangan SR, et al. Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macaca mulatta) with malignant lymphoma. Int. J. Cancer 38: 425-432, 1986.LCL 8664細胞 PubMed: 3017870