VA-ES-BJ細(xì)胞

年限： 41 years

數(shù)量： 大量

ATCC Number： CRL-2138?

相關(guān)**： 其他**

細(xì)胞形態(tài)： 上皮樣

生長狀態(tài)： 貼壁生長

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 1

物種來源： 人

Designations: VA-ES-BJ

VA-ES-BJ細(xì)胞Depositors: C Helson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Disease: epithelioid carcinoma

Derived from metastatic site: bone marrow

Cellular Products: granulocyte macrophage colony stimulating factor (GM-CSF)

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Tumorigenic: Yes

Oncogene:VA-ES-BJ細(xì)胞 p53 +

Cytogenetic Analysis: near triploid karyotype with structural as well as numerical abnormalities; modal number = 65. Rearrangements of both chromosomes 1 and 9 with unknown materials were present., Five copies of chromosome 7 were seen in almost every cell, and there was no intact Y chromosome present. Cell to cell variation was due to numerical differences only, structural abnormalities were very consistent.

Age: 41 years

Gender: male

Ethnicity: Caucasian

Comments: VA-ES-BJ is an epithelioid sarcoma cell line derived in 1991 from a bone marrow metastasis removed from an ***** male with primary vertebral epithelioid carcinoma.

The cells are sensitive to hemin.

The cell are positive for immunohistochemical staining for the following: AE-1 and AE-3 cytokeratins, fas, epithelial membrane antigen (EMA), vimentin and p53.

VA-ES-BJ細(xì)胞Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.1 mM non-essential amino acids; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended

Medium Renewal: Twice per week

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22334: . . Int. J. VA-ES-BJ細(xì)胞Oncol. 7: 51-56, 1995.